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Biochemical Applications of Gas Chromatography
This book was written to fulfill a need for a text on gas chromatography that would supply both the theoretical background and details of the appli- cations of this technique to biochemical problems. Several excellent books exist which review theory and instrumentation, but these do not contain sufficient experimental detail to be useful as laboratory manuals. On the other hand, the field has not matured sufficiently to make it possible to present a set of routine methods that can be used with confidence in every application. Therefore, experimental details of the most promising proce- dures are set forth together with enough theoretical background to enable the experimentalist to improvise adaptations without an extensive study of the literature.
Material is included that will be of interest to analytical biochemists working in a variety of fields. These fields include foods, essential oils, amino acids, carbohydrates, pesticides, clinical chemistry, and others. Although the book is organized along biochemical lines, information is included that will be of value to workers in other disciplines. For example, specific instruc- tions are given for the analysis of atmospheric gases and for the resolution of isomeric aliphatic and aromatic hydrocarbons. This information should be useful in the solution of problems in air pollution and petroleum chemistry. In fact, it was necessary to draw upon the literature from these fields to round out the text in areas where biochemical applications were few or lacking altogether.
This book contains methods for the analysis of the principal groups of compounds of biochemical interest. However, the gas chromatographic method is so versatile that the material could not be organized coherently according to the functional groups occurring in the various compounds. For example, alcohols, aldehydes, and esters often coexist in a sample and can frequently be resolved in a single operation. Classification according to vapor pressure is also possible, but this does not allow for a logical presentation of biochemical data. Therefore, the methods employed for sample collection and pre-fractionation are used as the primary basis for chapter organization, since this permits an orderly arrangement of the material from the viewpoint of experimental techniques. Thus volatile components of tissues, essential oils, lipids, and nonvolatile components of tissues, are each treated as distinct groups, since the methods used for sample preparation within each group are similar. The chapters are then arranged in approximate order of decreasing vapor pressure of the compounds discussed in them, and subdivisions within chapters made according to elements and functional groups where this is
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